The double-stranded RNA genome segments of cytoplasmic polyhedrosis virus are independently transcribed
نویسندگان
چکیده
منابع مشابه
Homologous Terminal Sequences in the Double-Stranded RNA Genome Segments of Cytoplasmic Polyhedrosis Virus of the Silkworm Bombyx mori.
The 3'-terminal regions (20 to 32 residues) of the genome double-stranded RNA (dsRNA) segments of cytoplasmic polyhedrosis virus were sequenced. The dsRNAs, which were labeled at their 3' termini by incubation with [5'-(32)P]pCp and T4 RNA ligase, were denatured and resolved into the plus and minus strands by agarose-urea gel electrophoresis. Ten single-stranded RNAs thus obtained from the five...
متن کاملDouble-stranded Ribonucleic Acid from Cytoplasmic Polyhedrosis Virus of the Silkworm.
Ribonucleic acid (RNA) was extracted by phenol treatment from cytoplasmic polyhedrosis virus isolated from the midgut of infected silkworms. This RNA appears as threads when precipitated in alcohol. Two components having different sedimentation constants were observed. The molecular weight of the RNA preparation obtained by sedimentation coefficient (weight-averaged) and intrinsic viscosity was...
متن کاملVisualization of protein-RNA interactions in cytoplasmic polyhedrosis virus.
Unlike the multiple-shelled organization of other Reoviridae members, cytoplasmic polyhedrosis virus (CPV) has a single-shelled capsid. The three-dimensional structures of full and empty CPV by electron cryomicroscopy show identical outer shells but differ inside. The outer surface reveals a T=1 icosahedral shell decorated with spikes at its icosahedral vertices. The internal space of the empty...
متن کاملCytoplasmic Polyhedrosis Virus of Bombyx mori
Two structurally different classes of oligonucleotides accumulate in vitro in cytoplasmic polyhedrosis virus (CPV) transcription mixtures in molar excess as compared to the completed RNA products. The first class consists of oligonucleotides which correspond to the 5’terminal sequence of the virus mRNAs (referred to as initiator oligonucleotides). The major species of initiator oligonucleotides...
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Bluetongue virus (BTV) causes hemorrhagic disease in economically important livestock. The BTV genome is organized into ten discrete double-stranded RNA molecules (S1-S10) which have been suggested to follow a sequential packaging pathway from smallest to largest segment during virus capsid assembly. To substantiate and extend these studies, we have investigated the RNA sorting and packaging me...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1982
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.41.1.326-329.1982